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An automated Anvi'o v9 Snakemake workflow for competitive metagenomic read recruitment. Resolves conserved sequence homologies to enable strain-level taxonomic profiling, population-level abundance estimation, and genome detection limits across environmental samples.

  • Updated Jul 7, 2026
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A genome-resolved metagenomics pipeline replicating the Sharon et al. (2019) gut-brain axis study. Features MEGAHIT de novo co-assembly, Bowtie2 competitive read recruitment, and Anvi'o single-nucleotide variant (SNV) microdiversity profiling.

  • Updated Jul 11, 2026
  • Shell

This pipeline implements competitive fragment recruitment to map metagenomic sequence space against a representative reference genome. In microbial ecology, read recruitment provides a quantitative window into the evolutionary dynamics of natural populations by measuring nucleotide-level polymorphism and consensus sequence divergence.

  • Updated Jul 2, 2026
  • Shell

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