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PharmaGTEx

A community-driven, cross-species gene expression atlas for drug discovery.

Motivation

Comparing gene/transcript expression patterns across human, mouse, rat, and cynomolgus monkey (cyno) is a routine but critical step in target validation, translational safety assessment, and PK/PD modeling during drug discovery. Despite how often this comparison is needed, there is currently no public, unified dataset that lets a scientist query a single gene or transcript across all four species and all major organs/tissues at once.

Efforts like GTEx provide deep human tissue expression data, but there is no equivalent harmonized resource spanning the other three species commonly used in preclinical research, nor a resource that puts all four side by side.

Goal

Build and maintain an open, community-contributed compendium of RNA-seq TPM (transcripts per million) expression values for:

  • Species: human, mouse, rat, cynomolgus monkey
  • Coverage: as many organs/tissues as possible per species (e.g., brain, heart, liver, kidney, lung, muscle, skin, blood, spleen, testis, ovary, etc.)
  • Unit: gene- and transcript-level TPM, harmonized to a common gene/ortholog identifier scheme across species

The end result should let anyone answer: "How is gene X expressed across organs in human, mouse, rat, and cyno?" — in one query, from one place.

How to contribute

This project is a community collaboration and welcomes contributions from any scientist. Ways to help:

  1. Contribute data — Point us to or upload public RNA-seq TPM matrices (with proper source/license attribution) for any of the four species and any organ/tissue not yet covered.
  2. Contribute mapping/annotation — Help harmonize gene/transcript IDs and orthology mapping across species (e.g., Ensembl, NCBI, HomoloGene/Orthology resources).
  3. Contribute code — Scripts for data processing, QC, normalization, and the query interface are all welcome.
  4. Open an issue — Propose a dataset, report a data quality concern, or suggest a tissue/organ to prioritize.

Please open a pull request or issue to get started. All are welcome regardless of experience level.

Status

Early stage — dataset structure and contribution guidelines are still being established. Watch this repo for updates.

License

TBD (data contributions must be from sources compatible with open redistribution; please note the original source/license when contributing).

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Community-curated cross-species RNA-seq TPM expression atlas (human, mouse, rat, cynomolgus monkey) across organs/tissues for drug discovery

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