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3 changes: 3 additions & 0 deletions 01-02-setup.Rmd
Original file line number Diff line number Diff line change
Expand Up @@ -21,6 +21,7 @@ install.packages(c(
"gprofiler2",
"ggupset",
"ggtangle",
"ggridges", # needed by enrichplot::ridgeplot()
"msigdbr",
"ontologyIndex",
"WebGestaltR",
Expand All @@ -29,6 +30,8 @@ install.packages(c(
))
```

**Note on `WebGestaltR`:** it includes Rust source code that gets compiled during installation, so a working Rust toolchain (including `cargo`) is required beforehand. If installation fails with a "cargo command is not available" or similar error, install/update Rust via [rustup](https://www.rust-lang.org/tools/install) rather than an older distro-packaged `cargo`, then retry `install.packages("WebGestaltR")`.

<p>&nbsp;</p> <!-- insert blank line -->

## Bioconductor packages
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2 changes: 2 additions & 0 deletions 08-2-stringdb.Rmd
Original file line number Diff line number Diff line change
Expand Up @@ -215,6 +215,8 @@ sema3a <- string_db$mp("sema3a")
string_db$get_interactions(c(crabp2, sema3a))
```

An empty data frame here is expected, not an error: `CRABP2` and `SEMA3A` are not annotated as directly interacting in STRING, so there is no row to return. `get_interactions` only reports edges that exist directly between the queried proteins; it will not tell you about shared partners or indirect paths between them.

### Interaction partners

To retrieve the first-shell interaction partners of one or more proteins from the locally loaded network, use `get_interaction_partners`. The output has the same structure as `get_interactions`.
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