reactomics.bib

@article{hu_tracking_2023,
	title = {Tracking the transformation pathway of dissolved organic matters ({DOMs}) in biochars under sludge pyrolysis via reactomics and molecular network analysis},
	url = {https://pubmed.ncbi.nlm.nih.gov/37709065/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1016/j.chemosphere.2023.140149},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Chemosphere. 2023 Nov;342:140149. doi: 10.1016/j.chemosphere.2023.140149. Epub 2023 Sep 12.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}This work examined the transformation pathways of sludge biochar-derived dissolved organic matters (SBC-derived DOMs) under sludge pyrolysis via FT-ICR-MS-based reactomics and molecular network analysis. Lignin/carboxylic-rich alicyclic molecules, proteins/aliphatic, and lipids of SBC-derived DOMs did not contribute equally to the overall pyrolytic reactions. Reactomics suggested that the pyrolysis reactions of SBC-derived DOMs consist of multiple cascade reactions involving the elimination of assemblages of reactive fragments during each pyrolysis reaction region, and the overall pyrolysis process was divided into three stages according to cascade reaction variations. Especially, cascade reactions at 400-500 °C produced potential environmental risk substances of N-containing, carbonyl-containing, and phenolic compounds. Besides, network analysis unraveled the complexity and number of molecular reaction pairs of SBC-derived DOMs decreased with the increase in pyrolytic temperatures. Keystone molecules and pathways results indicated that the pyrolytic temperature of the sludge pyrolysis process should be controlled at temperatures above 500 °C according to the harmful substances generation pattern in reaction products. Overall, the possible transformation pathways of SBC-derived DOMs during sludge pyrolysis treatment were proposed. This study elucidated the underlying mechanisms in generating SBC-derived DOMs and provided theoretical support for process optimization and harmful substances control of sludge pyrolysis.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/37709065/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}37709065{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1016/j.chemosphere.2023.140149"{\textgreater}10.1016/j.chemosphere.2023.140149{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Chemosphere},
	author = {Hu, Aibin and Zheng, Yongliang and Wang, Zheng and Li, Mengqiu and Wang, Dongsheng and Zhang, Weijun},
	month = sep,
	year = {2023},
}

@article{dwinandha_interpretable_2023,
	title = {Interpretable {Machine} {Learning} and {Reactomics} {Assisted} {Isotopically} {Labeled} {FT}-{ICR}-{MS} for {Exploring} the {Reactivity} and {Transformation} of {Natural} {Organic} {Matter} during {Ultraviolet} {Photolysis}},
	url = {https://pubmed.ncbi.nlm.nih.gov/38111239/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1021/acs.est.3c05213},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Environ Sci Technol. 2024 Jan 9;58(1):816-825. doi: 10.1021/acs.est.3c05213. Epub 2023 Dec 18.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Isotopically labeled FT-ICR-MS combined with multiple post-analyses, including interpretable machine learning (IML) and a paired mass distance (PMD) network, was employed to unravel the reactivity and transformation of natural organic matter (NOM) during ultraviolet (UV) irradiation. FT-ICR-MS analysis was used to assign formulas, which were classified on the basis of their molecular compositions and structural categories. Isotope (deuterium, D) labeling was utilized to unequivocally determine the photochemical products and examine the development of OD radical-mediated NOM transformation. With regard to the reactive molecular formulas, CHOS formulas exhibited the highest reactivity (86.5\% of precursors disappeared) followed by CHON (53.4\%) and CHO (24.6\%) formulas. With regard to structural categories, the degree of reactivity decreased in the following order: tannins \> condensed aromatics \> lignin/CRAMs. The IML algorithm demonstrated that the crucial features governing the reactivity of formulas were the molecular weight, DBE-O, NOSC, and the presence of heteroatoms (i.e., N and S), suggesting that the large and unsaturated compounds containing S and N are more prone to photodegradation. The reactomics approach using the PMD network further indicated that 11 specific molecular formulas in the CHOS and CHO class served as hubs, implying a higher photoreactivity and participation in a range of transformations. The isotope labeling analyses also found that, among the reactions observed, hydroxylation (i.e., +OD) is dominant for lignin/CRAMs and condensed aromatics, and formulas containing ≤10 D atoms were developed. Overall, this study, by adopting rigorous and interpretable techniques, could provide in-depth insights into the molecular-level dynamics of NOM under UV irradiation.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/38111239/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}38111239{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1021/acs.est.3c05213"{\textgreater}10.1021/acs.est.3c05213{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Environmental science \& technology},
	author = {Dwinandha, Dhimas and Elsamadony, Mohamed and Gao, Rongjun and Fu, Qing-Long and Liu, Jibao and Fujii, Manabu},
	month = dec,
	year = {2023},
}

@article{chen_inhibitory_2024,
	title = {Inhibitory effect of microplastics derived organic matters on humification reaction of organics in sewage sludge under alkali-hydrothermal treatment},
	url = {https://pubmed.ncbi.nlm.nih.gov/38324988/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1016/j.watres.2024.121231},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Water Res. 2024 Mar 15;252:121231. doi: 10.1016/j.watres.2024.121231. Epub 2024 Jan 29.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Alkali-hydrothermal treatment (AHT) of sewage sludge is often used to recover value-added dissolved organic matters (DOM) enriched with artificial humic acids (HA). Microplastics (MPs), as emerging contaminants in sewage sludge, can leach organic compounds (MP-DOM) during AHT, which potentially impact the characteristics of thermally treated sludge's DOM. This study employed spectroscopy and Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR-MS) to explore the impacts of MPs on DOM composition and transformation during AHT. The biological effects of DOM were also investigated by hydroponic experiments. The results showed that the leaching of MP-DOM led to a substantial increase in DOC content of DOM of thermally treated sludge. Conversely, the HA content significantly decreased in the presence of MPs, resulting in a decline of plant growth facilitation degree. FT-ICR-MS analysis revealed that the reduction in HA content was characterized by a notable decline in the abundance of O$_{\textrm{6-7}}$ and N$_{\textrm{1-3}}$O$_{\textrm{6-7}}$ molecules. Reactomics results indicated that the leaching of MP-DOM inhibited the Maillard reaction but bolstered oxidation reactions. The inhibition of Maillard reaction, resulting in a decrease in crucial precursors (dicarbonyl compounds, ketoses, and deoxyglucosone), was responsible for the decrease of HA content. The primary mechanism responsible for inhibiting the Maillard reaction was the consumption of reactive amino reactants through two pathways. Firstly, the leaching of organic acids in MP-DOM caused decrease of sludge pH, leading to the protonation of amino groups. Secondly, the lipid-like compounds in MP-DOM underwent oxidation (-2H+O), producing fatty aldehydes that consumed the reactive amino reactants. These discoveries offer enhanced insights into the specific contribution of MPs to the composition, transformation, bioactivity of DOM during AHT process.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/38324988/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}38324988{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1016/j.watres.2024.121231"{\textgreater}10.1016/j.watres.2024.121231{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Water research},
	author = {Chen, Jun and Sun, Tong and Yang, Peng and Peng, Siwei and Yu, Junxia and Wang, Dongsheng and Zhang, Weijun},
	month = feb,
	year = {2024},
}

@article{hu_complexation_2024,
	title = {Complexation with {Metal} {Ions} {Affects} {Chlorination} {Reactivity} of {Dissolved} {Organic} {Matter}: {Structural} {Reactomics} of {Emerging} {Disinfection} {Byproducts}},
	url = {https://pubmed.ncbi.nlm.nih.gov/39042037/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1021/acs.est.4c03022},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Environ Sci Technol. 2024 Aug 6;58(31):13890-13903. doi: 10.1021/acs.est.4c03022. Epub 2024 Jul 23.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Metal ions are liable to form metal-dissolved organic matter [dissolved organic matter (DOM)] complexes, changing the chemistry and chlorine reactivity of DOM. Herein, the impacts of iron and zinc ions (Fe$^{\textrm{3+}}$ and Zn$^{\textrm{2+}}$) on the formation of unknown chlorinated disinfection byproducts (Cl-DBPs) were investigated in a chlorination system. Fe$^{\textrm{3+}}$ preferentially complexed with hydroxyl and carboxyl functional groups, while Zn$^{\textrm{2+}}$ favored the amine functional groups in DOM. As a consequence, electron-rich reaction centers were created by the C-O-metal bonding bridge, which facilitated the electrophilic attack of α-C in metal-DOM complexes. Size-reactivity continuum networks were constructed in the chlorination system, revealing that highly aromatic small molecules were generated during the oxidation and decarbonization of metal-DOM complexes. Molecular transformation related to C-R (R represents complex sites) loss was promoted via metal complexation, including decarboxylation and deamination. Consequently, complexation with Fe$^{\textrm{3+}}$ and Zn$^{\textrm{2+}}$ promoted hydroxylation by the C-O-metal bonding bridge, thereby increasing the abundances of unknown polychlorinated Cl-DBPs by 9.6 and 14.2\%, respectively. The study provides new insights into the regulation of DOM chemistry and chlorine reactivity by metal ions in chlorination systems, emphasizing that metals increase the potential health risks of drinking water and more scientific control standards for metals are needed.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/39042037/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}39042037{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1021/acs.est.4c03022"{\textgreater}10.1021/acs.est.4c03022{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Environmental science \& technology},
	author = {Hu, Qian and Lou, Mingxuan and Wang, Ruigang and Bai, Sai and Guo, He and Zhou, Jian and Ma, Qiuling and Wang, Tiecheng and Zhu, Lingyan and Zhang, Xiangru},
	month = jul,
	year = {2024},
}

@article{chen_revealing_2024,
	title = {Revealing the interplay of dissolved organic matters variation with microbial symbiotic network in lime-treated sludge landscaping},
	url = {https://pubmed.ncbi.nlm.nih.gov/39442659/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1016/j.envres.2024.120216},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Environ Res. 2024 Dec 15;263(Pt 3):120216. doi: 10.1016/j.envres.2024.120216. Epub 2024 Oct 22.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Lime pretreatment is commonly used for sludge hygienization. Appropriate lime dosage is crucial for achieving both sludge stabilization (lime dosage \>0.2 g/g-TS) and promoting plant and soil health during subsequent landscaping (lime dosage \<0.8 g/g-TS). While much research has been conducted on sludge lime treatment, few studies have examined the effects of lime dosing on integrating sludge stabilization and plant growth promotion during landscaping. In this study, we investigated microbial dynamics and dissolved organic matter (DOM) transformation during sludge landscaping with five lime dosage gradients (0, 0.2, 0.4, 0.6, 0.8 g lime/g-TS) over 90 days. Our results showed that a lime dosage of 0.4 g/g-TS is the lower threshold for achieving waste activated sludge (WAS) stabilization during landscaping, leading to maximum humic substance formation and minimal phytotoxicity. Specifically, at 0.4 g/g-TS lime dosage, protein degradation and decarboxylation-induced humification were significantly enhanced. The predominant microbial genera shifted from Aromatoleum to Exiguobacterium and Romboutsia (both affiliated with the phylum Firmicutes). Reactomics analysis further indicated that a 0.4 g/g-TS lime dosage promoted the hydrolysis of proteins (lyase reactions on C-C, C-O, and C-N bonds), amino acid metabolism, and decarboxylation-induced humification (e.g., C$_{\textrm{1}}$H$_{\textrm{2}}$O$_{\textrm{2}}$, C$_{\textrm{2}}$H$_{\textrm{4}}$O$_{\textrm{2}}$, C$_{\textrm{5}}$H$_{\textrm{4}}$O$_{\textrm{2}}$, C$_{\textrm{6}}$H$_{\textrm{4}}$O$_{\textrm{2}}$). The co-occurrence network analysis suggested that the phyla Firmicutes, Proteobacteria, and Bacteroidetes were key players in DOM transformation. This study provides an in-depth understanding of microbe-mediated DOM transformation during sludge landscaping and identifies the optimal lime dosage for improving sludge landscaping efficiency.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/39442659/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}39442659{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1016/j.envres.2024.120216"{\textgreater}10.1016/j.envres.2024.120216{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Environmental research},
	author = {Chen, Zexu and Zhang, Yu and Yang, Boyuan and Fan, Sen and Li, Lanfeng and Yang, Peng and Zhang, Weijun},
	month = oct,
	year = {2024},
}

@article{ali_unveiling_2025,
	title = {Unveiling molecular {DOM} reactomics and transformation coupled with multifunctional nanocomposites under anaerobic conditions: {Tracking} potential metabolomics and pathways},
	url = {https://pubmed.ncbi.nlm.nih.gov/39837067/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1016/j.chemosphere.2025.144111},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Chemosphere. 2025 Mar;372:144111. doi: 10.1016/j.chemosphere.2025.144111. Epub 2025 Jan 20.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Anaerobic digestion (AD) offers great potential for pollutant removal and bioenergy recovery. However, it faces challenges when using livestock manure (LSM) as a feedstock given its high content of refractory materials (e.g., lignocellulose, long-chain carbohydrates, lipids, and crude protein). This would significantly inhibit AD-microbial activities, reduce organic transformation efficiency and limit gas production. To overcome this, multifunctional metal-doped hydrochars (HCs) were introduced here as AD supplements/accelerators, given that LSM degradation under AD results in complex dissolved organic matter (DOM). To assess this, the current study investigates the molecular interactions/transformations within DOM during LSM-AD coupled with metal-doped HCs, via batch-mode experiments. Expansive data mining techniques were employed to analyze DOM using Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Substantial increments in peptide-like along with decrements in highly unsaturated-like molecules were observed in HC@MnCl$_{\textrm{2}}$ containing-system. This indicates an increased capability for substrate hydrolysis and potential utilization of soluble microbial products (SMPs) (i.e., highly unsaturated-like molecules), leading to enhanced methane recovery (223.23 mL/g-VS$_{\textrm{added}}$, 1.77 times more than the control). However, accumulation of DOM-highly unsaturated molecules (i.e., a lack of SMPs' degradation) accompanied with low methane production (39.68 mL/g-VS$_{\textrm{added}}$) was noticed for HC@NiFe$_{\textrm{2}}$O$_{\textrm{4}}$. DOM reactivity during LSM-AD was validated via paired mass difference molecular network, indicating predominance of CHO and N-containing groups' transformations for HC@MnCl$_{\textrm{2}}$ and HC@NiFe$_{\textrm{2}}$O$_{\textrm{4}}$, respectively. Potential metabolites and abundant pathways were verified via KEGG database. This study improves our understanding of LSM-AD-DOM complex transformation matrix, the fate of bioavailable/recalcitrant compounds, and identification of potential DOM regulators from thousands of molecules.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/39837067/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}39837067{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1016/j.chemosphere.2025.144111"{\textgreater}10.1016/j.chemosphere.2025.144111{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Chemosphere},
	author = {Ali, Manal and Liu, Jibao and Kwon, Eunsang and Fujii, Manabu},
	month = jan,
	year = {2025},
}

@article{cao_enhanced_2025,
	title = {Enhanced {Release} and {Reactivity} of {Soil} {Water}-{Extractable} {Organic} {Matter} {Following} {Wildfire} in a {Subtropical} {Forest}},
	url = {https://pubmed.ncbi.nlm.nih.gov/39982015/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1021/acs.est.4c13557},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Environ Sci Technol. 2025 Mar 4;59(8):3992-4002. doi: 10.1021/acs.est.4c13557. Epub 2025 Feb 21.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Climate-driven increases in wildfire frequency may disrupt soil carbon dynamics, potentially creating positive feedback within global carbon cycle. However, the release and lability of soil carbon following wildfire remain unclear, limiting our ability to predict fire impacts on carbon cycling. Here, we investigated chemical alterations in soil water-extractable organic matter (WEOM) following a subtropical forest wildfire by comparing burned soils to an adjacent unburned site. The consensus is that fire-altered DOM is aromatic and less reactive. However, we found that 10 months postfire, burned soils contained nearly three times more water-extractable organic carbon (WEOC) than the control site. Reactomics analysis further revealed an overall 8-fold increase in potential reactivity of this carbon, identified by higher abundances of molecular formulas involved in identified microbial reaction pathways. Specifically, burned soils exhibited elevated potential oxidative enzyme reactions, linked to a higher nominal oxidation state of carbon (NOSC) in WEOM. Metagenomic analysis revealed an enrichment of microbial taxa specialized in degrading aromatic compounds in burned areas, supporting the occurrence of potential microbial reaction pathways acting on WEOM in postfire soils. These findings highlight that wildfires may accelerate soil carbon loss through reactive WEOM mobilization and microbial response, with implications for long-term carbon-climate projections.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/39982015/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}39982015{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1021/acs.est.4c13557"{\textgreater}10.1021/acs.est.4c13557{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Environmental science \& technology},
	author = {Cao, Xinghong and Ma, Hua and Li, Sheng-Ao and Huang, Hai and Cui, Fuyi and Tanentzap, Andrew J.},
	month = feb,
	year = {2025},
}

@article{cao_wildfire-derived_2025,
	title = {Wildfire-{Derived} {Pyrogenic} {Dissolved} {Organic} {Matter} ({pyDOM}) {Enhances} {Riverine} {DOM} {Reactivities} and {Nitrogen} {Metabolisms}},
	url = {https://pubmed.ncbi.nlm.nih.gov/40481773/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1021/acs.est.5c01794},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Environ Sci Technol. 2025 Jun 17;59(23):11597-11606. doi: 10.1021/acs.est.5c01794. Epub 2025 Jun 7.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Wildfires profoundly reshape soil organic matter composition with cascading impacts on global carbon cycling, yet, the biogeochemical consequences of pyrogenic dissolved organic matter (pyDOM) on riverine DOM reactivity and microbial metabolism remain poorly constrained. Here, we conducted controlled incubation of river water amended with DOM extracted from wildfire-affected versus undisturbed soils to assess molecular DOM transformations and microbial responses. High-resolution mass spectrometry and substrate-explicit modeling revealed that pyDOM introduction increased refractory components (e.g., condensed aromatics and tannins) with high modified aromaticity index (AI$_{\textrm{mod}}$) and double bond equivalents (DBE). Reactomics analysis revealed that pyDOM exhibited enhanced reactivity which may be associated with alterations in macromolecular electron shuttles and water solubility. While pyDOM introduction reduced overall riverine microbial diversity and abundance, it triggered a 17-fold increase of filamentous cyanobacteria abundance, simultaneously boosting both autotrophic capabilities and the functional abundance related to nitrogen metabolism in riverine microorganisms. Genomic evidence from PICRUSt2 analysis demonstrated pyDOM-driven enrichment of denitrification pathways, particularly through upregulation of periplasmic nitrate reductase components (\textit{napA} + 3.0-fold; \textit{napB} + 3.1-fold), suggesting enhanced aerobic denitrification capacity. These findings establish pyDOM as a biogeochemical vector that redirects terrestrial carbon sequestration into aquatic metabolic network, emphasizing the need to integrate pyDOM fluxes into climate-relevant biogeochemical frameworks.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/40481773/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}40481773{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1021/acs.est.5c01794"{\textgreater}10.1021/acs.est.5c01794{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Environmental science \& technology},
	author = {Cao, Mingxing and Ma, Hua and Ye, Yixuan and Li, Sheng-Ao and Cao, Xinghong and Huang, Hai and Li, Zhe and Cui, Fuyi},
	month = jun,
	year = {2025},
}

@article{zhang_decoding_2025,
	title = {Decoding periodate-driven phototransformation of dissolved organic matter in sunlit waters: {Multidimensional} property shifts and molecular network reconfiguration},
	url = {https://pubmed.ncbi.nlm.nih.gov/40769065/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1016/j.watres.2025.124331},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Water Res. 2025 Dec 1;287(Pt A):124331. doi: 10.1016/j.watres.2025.124331. Epub 2025 Aug 5.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Residual periodate (PI) from advanced oxidation processes poses emerging threats to aquatic carbon cycling, yet its role in reprogramming the phototransformation of dissolved organic matter (DOM) remains poorly understood. Here, we reveal the multidimensional effects of PI residues (20.0-300.0 μM) on DOM under solar irradiation by real-time photophysical, electrochemical, and photochemical tracking; molecular reactomics via FT-ICR MS with paired mass distance networks; and interpretable machine learning. PI residues induced aromatic structure fragmentation, reductions in apparent molecular weight, and increases in redox capacity by 1.4-3.6 times. The solar/PI system enhanced photochemical reactivity, elevating the quantum yields of $^{\textrm{3}}$DOM* (humic acid (HA): 0.50→0.75; Suwannee River HA (SRHA): 0.78→1.44) and $^{\textrm{•}}$OH (HA: 1.85→2.38; SRHA: 1.82→3.19) via quinone-mediated pathways. Propertied correlations varied depending on the DOM source. Molecular reactomics revealed PI-driven CN bond cleavage (18.18 \% increase in N-group removal) and the polymerisation of small molecules (e.g., tannins accumulated by 8.5 \%) in contrast to natural photoageing. SHAP analysis using XGBoost further highlighted the significance of nitrogen and bidirectional control of the O/C ratio. These findings redefine DOM transformation mechanisms in carbon-nitrogen cycling towards N-centred under anthropogenic oxidant stress, accelerating dissolved organic nitrogen production, and increasing the risk of nitrogenous disinfection by-products.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/40769065/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}40769065{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1016/j.watres.2025.124331"{\textgreater}10.1016/j.watres.2025.124331{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Water research},
	author = {Zhang, Kaiting and Zheng, Ruiqi and Li, Lihua and Jiang, Bin and Feng, Mingbao},
	month = aug,
	year = {2025},
}

@article{du_molecular_2025,
	title = {Molecular {Humification} {Mechanisms} of {Dissolved} {Organic} {Matter} during {Maize} {Straw} {Composting} {Enhanced} by {Humus} {Soil} {Biomaterial}: {Paired}-{Molecule} {Mass} {Difference} {Reactomics} {Analysis} {Based} on {FT}-{ICR} {MS}},
	url = {https://pubmed.ncbi.nlm.nih.gov/40811610/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1021/acs.jafc.5c05559},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}J Agric Food Chem. 2025 Aug 27;73(34):21504-21515. doi: 10.1021/acs.jafc.5c05559. Epub 2025 Aug 14.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}This study investigated molecular humification mechanisms of dissolved organic matter during maize straw composting enhanced by humus soil biomaterial using paired-molecule mass difference reactomics analysis and high-throughput sequencing. Results indicated that the composting process predominantly involved three molecular humification pathways, namely, the phenol-protein reaction, polyphenol self-condensation, and Maillard reaction, with N-containing molecules showing the highest reactivity. Proteins were hydrolyzed into N-containing intermediates in the initial composting stage, which rapidly polymerized with phenol from humus soil biomaterial to form humic acids, improving organic nitrogen retention. Lignin was decomposed and oxidized into phenolics and quinones mainly during the middle and later composting stages, which either self-condensed or polymerized with protein derivatives to assemble humic acids. Concurrently, some amino acids and monosaccharides underwent the Maillard reaction to produce humic acids. Furthermore, humus soil biomaterial introduced the genus \textit{Alicyclobacillus} into the composting system, contributing to microbial community stability. This work provides valuable molecular insights into the maize straw composting humification process and promotes the sustainable utilization of agricultural waste in soil.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/40811610/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}40811610{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1021/acs.jafc.5c05559"{\textgreater}10.1021/acs.jafc.5c05559{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Journal of agricultural and food chemistry},
	author = {Du, Zheng-Liang and Fu, Qing-Long and Liu, Jia-Yu and Chai, Xiao-Li and Wu, Bo-Ran},
	month = aug,
	year = {2025},
}

@article{liu_microbial_2025,
	title = {Microbial {Physiological} {Adaptation} to {Biodegradable} {Microplastics} {Drives} the {Transformation} and {Reactivity} of {Dissolved} {Organic} {Matter} in {Soil}},
	url = {https://pubmed.ncbi.nlm.nih.gov/40924621/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1021/acs.est.5c09633},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Environ Sci Technol. 2025 Sep 23;59(37):19856-19871. doi: 10.1021/acs.est.5c09633. Epub 2025 Sep 9.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}The turnover of dissolved organic matter (DOM) in soil regulated by biodegradable microplastics (MPs) has garnered much attention due to its profound impact on the storage and stability of soil organic matter. However, the transformation and reactivity of plant-derived and microbially derived DOM by microorganisms adapted to biodegradable MPs, and the involved microbial physiological processes, remain nearly unknown. Here, we added virgin and aged polylactic acid (PLA) and polyhydroxyalkanoate (PHA) to agricultural soils and incubated for 56 days. Using stable isotope techniques, reactomics, and metagenomics, we found that the addition of both virgin and aged PLA induced hydroxylation, demethylation, and dehydrogenation of lignin-derived DOM, resulting in a 3-fold increase in their oxidation degree. PLA activated the enzymatic pathway for lignin-derived DOM decomposition and downregulated genes involved in bacterial anabolism, such as those related to protein, amino sugar, and peptidoglycan biosynthesis. In contrast, PHA increased the content of microbially derived DOM compounds such as proteins and amino sugars by 2.1-fold relative to the control with peptide chain elongation. PHA resulted in the degradation of lignin-derived DOM into pyruvate and acetyl-CoA, accelerated bacterial ATP synthesis, the de novo biosynthesis of proteins and peptidoglycan, and cell renewal and death, thereby increasing PHA- and soil organic matter-derived microbial necromass carbon. Our study provides new insights into the impact of biodegradable MPs on soil DOM transformation and underscores the importance of the microbial physiological processes involved.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/40924621/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}40924621{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1021/acs.est.5c09633"{\textgreater}10.1021/acs.est.5c09633{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Environmental science \& technology},
	author = {Liu, Lin and Hu, Long and Kuzyakov, Yakov and Rillig, Matthias C. and Duan, Guilan and Wei, Gehong and Chen, Chun},
	month = sep,
	year = {2025},
}

@article{petrick_molecular_2025,
	title = {Molecular {Reactivity} in {Maternal} {Pregnancy} {Blood} and {Neonatal} {Dried} {Blood} {Spots} {Is} {Associated} with the {Risk} of {Pediatric} {Acute} {Lymphoblastic} {Leukemia}},
	url = {https://pubmed.ncbi.nlm.nih.gov/40932331/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1158/1055-9965.EPI-25-0801},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Cancer Epidemiol Biomarkers Prev. 2025 Nov 3;34(11):1979-1988. doi: 10.1158/1055-9965.EPI-25-0801.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}BACKGROUND: Most pediatric leukemia forms early in life, but early-life biology and biomarkers for screening remain undefined. We performed direct molecular measurements in neonatal dried blood spots (DBS) and maternal pregnancy serum to identify early-life biological signatures of pediatric acute lymphoblastic leukemia (ALL).{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}METHODS: In a nested case-control study design, we obtained mother-infant paired samples from second-trimester pregnancy serum and neonatal DBS from 122 children diagnosed with pediatric ALL and 122 matched cancer-free controls. Using liquid chromatography-high-resolution mass spectrometry, we performed untargeted metabolomics. The data-driven Reactomics approach was used to identify quantitative paired mass differences (qPMD) that represent molecular changes in the samples. We identified qPMDs associated with ALL risk and assessed linolenic acid and linoleic acid as potential ALL biomarkers.{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}RESULTS: Overall, the nine selected qPMDs in DBS were more strongly associated with ALL than the 16 qPMDs in maternal serum. Several of the selected qPMDs were highly correlated, suggesting that these qPMDs may represent biological reactivity hubs of metabolic pathways important in leukemogenesis. We also observed a suggestive positive but not significant association between linolenic acid and linoleic acid in the DBS of children diagnosed with ALL at 5 years or older (N = 13) and matched controls (N = 13).{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}CONCLUSIONS: Although biological interpretation of Reactomics analysis for clinical intervention is currently limited, our study supports the presence of molecular reaction changes in early life associated with later pediatric ALL.{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}IMPACT: Reactomics analysis revealed potential biomarkers in neonatal samples linked with later diagnosis of ALL.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/40932331/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}40932331{\textless}/a{\textgreater} {\textbar} PMC:{\textless}a href="https://www.ncbi.nlm.nih.gov/pmc/PMC12496411/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}PMC12496411{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1158/1055-9965.EPI-25-0801"{\textgreater}10.1158/1055-9965.EPI-25-0801{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Cancer epidemiology, biomarkers \& prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology},
	author = {Petrick, Lauren M. and Imani, Partow and Yu, Miao and Dolios, Georgia and Dudoit, Sandrine and Morimoto, Libby M. and Ma, Xiaomei and Wiemels, Joseph L. and Metayer, Catherine},
	month = sep,
	year = {2025},
}

@article{liu_real-world_2025,
	title = {Real-world aged microplastics exacerbate antibiotic resistance genes dissemination in anaerobic sludge digestion via enhancing microbial metabolite communication-driven pilus conjugative transfer},
	url = {https://pubmed.ncbi.nlm.nih.gov/41344131/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1016/j.watres.2025.125056},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Water Res. 2026 Feb 15;290:125056. doi: 10.1016/j.watres.2025.125056. Epub 2025 Nov 26.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}The dissemination of antibiotic resistance genes (ARGs) facilitated by coexisting microplastics (MPs) in the "source-sink" hotspots of waste activated sludge (WAS) raises great concern. Despite real-world MPs undergoing aging, whether and how naturally aged microplastics (AMPs) affect ARG dissemination during sludge treatment remains largely unknown. Herein, we systematically explored the evolved effects and underlying mechanisms of environmentally relevant MPs (0, 3, and 30 mg/kg TS) aging on ARG propagation in anaerobic sludge digestion via multi-omics analyses. Specifically, microplastic exposure increased total ARG abundance by 2.59-15.31 \% with enriched mobile genetic elements (MGEs, 0.22-16.71 \%). These effects were escalated at higher microplastic dosages and aging degrees. Mechanistically, metagenomic and metaproteomic analyses revealed the drivers for ARG amplification in the sludge digester evolved from the pristine microplastics (PMPs)-induced higher oxidative stress and membrane permeability to AMPs-induced higher multidrug efflux coupled with pilus-mediated conjugation. Subsequently, metagenomic binning identified key multidrug-resistant hosts of Sedimentibacter, Alicycliphilus, and Sulfuricurvum genera. Moreover, high-resolution metabolomics and reactomics network analysis uncovered that AMPs stimulated microbial metabolite turnover, particularly of nitrogenous and sulfurous compounds, and enhanced the complexity and communication frequency of molecular transformation networks centered on lignin and protein nodes, thereby promoting ARG exchange. Finally, Mantel tests reconfirmed that reactive oxygen species level (Mantel's r = 0.93, p = 0.04) and metabolite network connectivity (Mantel's r = 0.82, p = 0.04) are paramount drivers of ARG spread. These findings offer novel insights into the ARG amplification risk driven by MPs aging, guiding targeted strategies to mitigate ARG spread and improve resource recovery in sludge bioengineering systems.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/41344131/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}41344131{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1016/j.watres.2025.125056"{\textgreater}10.1016/j.watres.2025.125056{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Water research},
	author = {Liu, Tao and Li, Lipin and Chen, Junjie and Cai, Guiyuan and Meng, Fan and Jiao, Yimeng and Mao, Yuqing and Wang, Zifan and Zuo, Wei and Tian, Yu and Sun, Huihang},
	month = dec,
	year = {2025},
}

@article{tu_insights_2026,
	title = {Insights into {Contaminant} {Composition} {Variations} and {Reactomics} during {Wastewater} {Treatment} {Processes} {Based} on {Nontargeted} {Analysis} and {Paired} {Mass} {Distance}},
	url = {https://pubmed.ncbi.nlm.nih.gov/41540359/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1021/acs.est.5c14774},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Environ Sci Technol. 2026 Feb 17;60(6):5086-5096. doi: 10.1021/acs.est.5c14774. Epub 2026 Jan 15.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Understanding contaminant transformation during wastewater treatment is essential for pollution reduction and environmental risk mitigation. However, the highly diverse molecular composition of wastewater, arising from various emission sources, presents a major challenge to deciphering the underlying biochemical reactions. Herein, nontargeted analysis and paired mass distance (PMD) analysis were conducted in paired influent-effluent samples from 11 wastewater treatment plants to elucidate contaminant diversity and transformation reactions across different treatment processes. In total, 418 structural subclasses of contaminants were classified, among which amino acids, peptides, and analogues (AAPAs) were the most common. Although the total number of features, subclasses, and overall abundance decreased in effluent samples, chemical diversity did not show a consistent reduction. This finding was primarily due to the removal of contaminants such as AAPAs and the persistence of aromatic compounds such as benzenoids. Transformation reactions were highly conserved across biological processes, with methylation and demethylation occurring the most frequently. High-frequency PMDs were strongly associated with carbon-related transformations, which substantially altered the polarity of transformation products. Furthermore, transformation reactions were characterized by small mass shifts and intrastructural reactions, indicating that most contaminants did not undergo extensive structural transformations. This study provides insight into common patterns of contaminant removal across wastewater treatment processes, benefiting transformation product prediction and wastewater treatment process optimization.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/41540359/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}41540359{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1021/acs.est.5c14774"{\textgreater}10.1021/acs.est.5c14774{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Environmental science \& technology},
	author = {Tu, Keng and Liu, Yun and Chen, Xichao and Li, Wei and Xu, Zhao and Qia, Chen and Yu, Yunjiang},
	month = jan,
	year = {2026},
}

@article{guo_transformation_2026,
	title = {Transformation process and removal mechanism of {DOM} based on paired mass distance ({PMD}) analysis in the multi-stage biological contact oxidation process},
	url = {https://pubmed.ncbi.nlm.nih.gov/41747906/?utm_source=Firefox&utm_medium=rss&utm_campaign=pubmed-2&utm_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU&fc=20201001215015&ff=20260427102726&v=2.19.0.post6+133c1fe},
	doi = {10.1016/j.biortech.2026.134282},
	abstract = {{\textless}div xmlns="http://www.w3.org/1999/xhtml"{\textgreater}{\textless}p style="color: \#4aa564;"{\textgreater}Bioresour Technol. 2026 May;448:134282. doi: 10.1016/j.biortech.2026.134282. Epub 2026 Feb 24.{\textless}/p{\textgreater}{\textless}p{\textgreater}\textbf{ABSTRACT}{\textless}/p{\textgreater}{\textless}p xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:p1="http://pubmed.gov/pub-one"{\textgreater}Understanding the degradation pathways of dissolved organic matter (DOM) in sewage treatment is critical for optimizing DOM removal efficiency. While the multi-stage biological contact oxidation (MBCO) process is increasingly used as a promising emerging wastewater treatment technology, insights into its DOM transformation mechanisms remain limited. This study, for the first time, elucidates DOM transformation pathways in MBCO by integrating Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS), pairwise mass difference (PMD) networks, and microbial community profiling. Results show biological treatment significantly boosts DOM's molecular diversity, with detected molecules nearly tripling. During treatment, lignin's proportion in DOM rises from 11.6\% to 73.4\%, along with increased aromaticity and humification. PMD analysis reveals DOM transformation mainly occurs via intermolecular conversion of lignin or saturated compounds, with minimal elemental composition changes; functional groups involved (e.g., additions/losses) are mostly composed of C, H, and O. Notably, MBCO has unique DOM transformation pathways vs. conventional biological processes, including potential substitution reactions with 1-hydroxyethyl and acetyl groups-likely due to its distinct microbial community. Correlation analysis between 6 DOM-degrading microorganisms (Serratia, Klebsiella, Escherichia\_Shigella, Pseudomonas, Bacillus, Clostridium) and 70 PMDs (linked to 3 common DOM degradation pathways: Glycolysis/Gluconeogenesis; Glycine, serine and threonine metabolism; Amino sugar and nucleotide sugar metabolism) shows different microbes prefer specific pathways. These findings guide targeted microbial enhancement in MBCO to improve DOM removal efficiency.{\textless}/p{\textgreater}{\textless}p style="color: lightgray"{\textgreater}PMID:{\textless}a href="https://pubmed.ncbi.nlm.nih.gov/41747906/?utm\_source=Firefox\&utm\_medium=rss\&utm\_campaign=pubmed-2\&utm\_content=1jKIOHIm0MJiyjPydQN9MAVaqiituWTwUuAfmrz4q718Og7MsU\&fc=20201001215015\&ff=20260427102726\&v=2.19.0.post6+133c1fe"{\textgreater}41747906{\textless}/a{\textgreater} {\textbar} DOI:{\textless}a href="https://doi.org/10.1016/j.biortech.2026.134282"{\textgreater}10.1016/j.biortech.2026.134282{\textless}/a{\textgreater}{\textless}/p{\textgreater}{\textless}/div{\textgreater}},
	language = {en},
	journal = {Bioresource technology},
	author = {Guo, Yuchen and Sun, Zhen and Wang, Jieqiong and Tian, Yonglan and Liu, Jibao and Guo, Zhongyu and Hu, Aibin and Wu, Dongming and Zhang, Yunfei and Yang, Shaoxia and Niu, Junfeng},
	month = feb,
	year = {2026},
}

@article{yu2019structure,
	title = {Structure/reaction directed analysis for {LC}-{MS} based untargeted analysis},
	doi = {10.1016/j.aca.2018.10.062},
	journal = {Analytica Chimica Acta},
	author = {Yu, Miao and Olkowicz, Mariola and Pawliszyn, Janusz},
	month = mar,
	year = {2019},
}