diff --git a/.changeset/quiet-pumas-dance.md b/.changeset/quiet-pumas-dance.md
new file mode 100644
index 000000000000..a91805ee1859
--- /dev/null
+++ b/.changeset/quiet-pumas-dance.md
@@ -0,0 +1,5 @@
+---
+'@sveltejs/kit': patch
+---
+
+fix: allow handle functions composed with sequence to run without a request store
diff --git a/packages/kit/src/exports/hooks/sequence.js b/packages/kit/src/exports/hooks/sequence.js
index 892dc973fbd4..e1715085ae9e 100644
--- a/packages/kit/src/exports/hooks/sequence.js
+++ b/packages/kit/src/exports/hooks/sequence.js
@@ -1,9 +1,14 @@
 /** @import { Handle, RequestEvent, ResolveOptions } from '@sveltejs/kit' */
+/** @import { RecordSpan } from 'types' */
 import {
 	merge_tracing,
-	get_request_store,
+	try_get_request_store,
 	with_request_store
 } from '@sveltejs/kit/internal/server';
+import { noop_span } from '../../runtime/telemetry/noop.js';
+
+/** @type {RecordSpan} */
+const noop_record_span = ({ fn }) => fn(noop_span);
 
 /**
  * A helper function for sequencing multiple `handle` calls in a middleware-like manner.
@@ -80,11 +85,11 @@ import {
  */
 export function sequence(...handlers) {
 	const length = handlers.length;
-	if (!length) return ({ event, resolve }) => resolve(event);
 
 	return ({ event, resolve }) => {
-		const { state } = get_request_store();
-		return apply_handle(0, event, {});
+		const store = try_get_request_store();
+		const record_span = store ? store.state.tracing.record_span : noop_record_span;
+		return apply_handle(0, event, undefined);
 
 		/**
 		 * @param {number} i
@@ -93,51 +98,47 @@ export function sequence(...handlers) {
 		 * @returns {Promise<Response>}
 		 */
 		function apply_handle(i, event, parent_options) {
+			if (i === length) return resolve(event, parent_options);
+
 			const handle = handlers[i];
 
-			return state.tracing.record_span({
+			return record_span({
 				name: `sveltekit.handle.sequenced.${handle.name ? handle.name : i}`,
 				attributes: {},
 				fn: async (current) => {
 					const traced_event = merge_tracing(event, current);
-					return await with_request_store({ event: traced_event, state }, () =>
-						handle({
-							event: traced_event,
-							resolve: (event, options) => {
-								/** @type {ResolveOptions['transformPageChunk']} */
-								const transformPageChunk = async ({ html, done }) => {
-									if (options?.transformPageChunk) {
-										html = (await options.transformPageChunk({ html, done })) ?? '';
-									}
-
-									if (parent_options?.transformPageChunk) {
-										html = (await parent_options.transformPageChunk({ html, done })) ?? '';
-									}
-
-									return html;
-								};
+					return await with_request_store(
+						store && { event: traced_event, state: store.state },
+						() =>
+							handle({
+								event: traced_event,
+								resolve: (event, options) => {
+									/** @type {ResolveOptions} */
+									const merged = {
+										transformPageChunk:
+											options?.transformPageChunk || parent_options?.transformPageChunk
+												? async ({ html, done }) => {
+														if (options?.transformPageChunk) {
+															html = (await options.transformPageChunk({ html, done })) ?? '';
+														}
 
-								/** @type {ResolveOptions['filterSerializedResponseHeaders']} */
-								const filterSerializedResponseHeaders =
-									parent_options?.filterSerializedResponseHeaders ??
-									options?.filterSerializedResponseHeaders;
+														if (parent_options?.transformPageChunk) {
+															html =
+																(await parent_options.transformPageChunk({ html, done })) ?? '';
+														}
 
-								/** @type {ResolveOptions['preload']} */
-								const preload = parent_options?.preload ?? options?.preload;
+														return html;
+													}
+												: undefined,
+										filterSerializedResponseHeaders:
+											parent_options?.filterSerializedResponseHeaders ??
+											options?.filterSerializedResponseHeaders,
+										preload: parent_options?.preload ?? options?.preload
+									};
 
-								return i < length - 1
-									? apply_handle(i + 1, event, {
-											transformPageChunk,
-											filterSerializedResponseHeaders,
-											preload
-										})
-									: resolve(event, {
-											transformPageChunk,
-											filterSerializedResponseHeaders,
-											preload
-										});
-							}
-						})
+									return apply_handle(i + 1, event, merged);
+								}
+							})
 					);
 				}
 			});
diff --git a/packages/kit/src/exports/hooks/sequence.spec.js b/packages/kit/src/exports/hooks/sequence.spec.js
index 7fe3afa49bdc..df8e889c0323 100644
--- a/packages/kit/src/exports/hooks/sequence.spec.js
+++ b/packages/kit/src/exports/hooks/sequence.spec.js
@@ -1,31 +1,23 @@
 /** @import { RequestEvent } from '@sveltejs/kit' */
-/** @import { RequestState } from 'types' */
-import { assert, expect, test, vi } from 'vitest';
+import { assert, expect, test } from 'vitest';
 import { sequence } from './sequence.js';
-import { noop_span } from '../../runtime/telemetry/noop.js';
-
-const dummy_event = vi.hoisted(
-	() =>
-		/** @type {RequestEvent} */ ({
-			tracing: {
-				root: {}
-			}
-		})
-);
-
-vi.mock(import('@sveltejs/kit/internal/server'), async (actualPromise) => {
-	const actual = await actualPromise();
-	return {
-		...actual,
-		get_request_store: () => ({
-			event: dummy_event,
-			state: /** @type {RequestState} */ ({
-				tracing: {
-					record_span: ({ fn }) => fn(noop_span)
-				}
-			})
-		})
-	};
+
+const dummy_event = /** @type {RequestEvent} */ ({
+	tracing: {
+		root: {}
+	}
+});
+
+test('runs without a request store', async () => {
+	const handler = sequence(
+		async ({ event, resolve }) => resolve(event),
+		async ({ event, resolve }) => resolve(event)
+	);
+
+	const response = new Response();
+	const event = /** @type {RequestEvent} */ ({});
+
+	assert.equal(await handler({ event, resolve: () => Promise.resolve(response) }), response);
 });
 
 test('applies handlers in sequence', async () => {