diff --git a/instrumentation/opentelemetry-instrumentation-sqlalchemy/src/opentelemetry/instrumentation/sqlalchemy/engine.py b/instrumentation/opentelemetry-instrumentation-sqlalchemy/src/opentelemetry/instrumentation/sqlalchemy/engine.py
index 6164403bcd..b2a798f4bd 100644
--- a/instrumentation/opentelemetry-instrumentation-sqlalchemy/src/opentelemetry/instrumentation/sqlalchemy/engine.py
+++ b/instrumentation/opentelemetry-instrumentation-sqlalchemy/src/opentelemetry/instrumentation/sqlalchemy/engine.py
@@ -3,6 +3,7 @@
 import os
 import re
 import weakref
+from sqlalchemy.exc import InvalidRequestError
 
 import sqlalchemy
 from sqlalchemy.event import (  # pylint: disable=no-name-in-module
@@ -308,15 +309,14 @@ def _register_event_listener(cls, target, identifier, func, *args, **kw):
 
     @classmethod
     def remove_all_event_listeners(cls):
-        for (
-            weak_ref_target,
-            identifier,
-            func,
-        ) in cls._remove_event_listener_params:
-            # Remove an event listener only if saved weak reference points to an object
-            # which has not been garbage collected
-            if weak_ref_target() is not None:
-                remove(weak_ref_target(), identifier, func)
+        for weak_ref_target, identifier, fn in cls._remove_event_listener_params:
+            target = weak_ref_target()
+            if target is None:
+                continue
+            try:
+                event.remove(target, identifier, fn)
+            except InvalidRequestError:
+                pass
         cls._remove_event_listener_params.clear()
 
     def _operation_name(self, db_name, statement):