diff --git a/aws-healthomics/POWER.md b/aws-healthomics/POWER.md
index 81a8754..16f66ed 100644
--- a/aws-healthomics/POWER.md
+++ b/aws-healthomics/POWER.md
@@ -27,6 +27,7 @@ Whenever you are asked to perform a task related to any of the following scenari
 - Setting up VPC infrastructure for HealthOmics workflows (subnets, NAT Gateway, security groups, endpoints) -> use `./steering/vpc-setup.md`
 - Managing HealthOmics VPC configurations (creating, listing, getting, or deleting configurations) -> use `./steering/healthomics-configuration.md`
 - Running workflows with VPC connectivity, public internet access, cross-region access, or access to private VPC resources -> use `./steering/vpc-connected-workflow-runs.md`
+- Understanding regional feature availability, GPU instance limitations, or troubleshooting region-specific errors -> use `./steering/regional-capabilities.md`
 
 
 # Onboarding
diff --git a/aws-healthomics/steering/regional-capabilities.md b/aws-healthomics/steering/regional-capabilities.md
new file mode 100644
index 0000000..5c872b9
--- /dev/null
+++ b/aws-healthomics/steering/regional-capabilities.md
@@ -0,0 +1,93 @@
+# Regional Capabilities and Limitations
+
+## Purpose
+
+This document describes region-specific capabilities and limitations for AWS HealthOmics. Not all HealthOmics features are available in all regions.
+
+## Authoritative Source
+
+For the most up-to-date information on supported regions, instance types, and features, refer to the [AWS HealthOmics public documentation](https://docs.aws.amazon.com/omics/latest/dev/memory-and-compute-tasks.html) and the [HealthOmics endpoints and quotas page](https://docs.aws.amazon.com/general/latest/gr/healthomics-quotas.html). IF the information in this document appears inconsistent with what public documentation states, defer to the public documentation.
+
+## When to Reference This Document
+
+Reference this document when:
+- The user asks about feature availability in a specific region
+- The user encounters errors related to Storage (sequence/reference stores) or Analytics (variant/annotation stores)
+- The user is choosing which region to deploy workflows in
+- The user asks about GPU instance type availability
+
+## Regional Feature Availability
+
+### Full-Feature Regions
+
+The following regions support **all** HealthOmics capabilities (Workflows, Storage, and Analytics):
+
+| Region | Region Code | Workflows | Storage | Analytics |
+|---|---|---|---|---|
+| US West (Oregon) | us-west-2 | Yes | Yes | Yes |
+| US East (N. Virginia) | us-east-1 | Yes | Yes | Yes |
+| Europe (Ireland) | eu-west-1 | Yes | Yes | Yes |
+| Europe (London) | eu-west-2 | Yes | Yes | Yes |
+| Europe (Frankfurt) | eu-central-1 | Yes | Yes | Yes |
+| Israel (Tel Aviv) | il-central-1 | Yes | Yes | Yes |
+| Asia Pacific (Singapore) | ap-southeast-1 | Yes | Yes | Yes |
+
+**All features include**:
+- Private workflows (custom WDL, Nextflow, CWL)
+- Ready2Run workflows (pre-built sample workflows)
+- Sequence stores and Reference stores (HealthOmics Storage)
+- Variant stores and Annotation stores (HealthOmics Analytics)
+- ECR Pull-Through Cache support
+
+### Workflows-Only Regions
+
+The following regions support **Workflows only** (no Storage or Analytics):
+
+| Region | Region Code | Workflows | Storage | Analytics |
+|---|---|---|---|---|
+| US East (Ohio) | us-east-2 | Yes | No | No |
+| Asia Pacific (Tokyo) | ap-northeast-1 | Yes | No | No |
+| Asia Pacific (Seoul) | ap-northeast-2 | Yes | No | No |
+
+**Supported features**:
+- Private workflows (custom WDL, Nextflow, CWL)
+- All standard CPU instance types
+- GPU instance types (availability varies — see below)
+- VPC networking and Configurations
+- ECR Pull-Through Cache
+
+**Not available in these regions**:
+- Ready2Run workflows
+- Sequence stores (HealthOmics Storage)
+- Reference stores (HealthOmics Storage)
+- Variant stores (HealthOmics Analytics)
+- Annotation stores (HealthOmics Analytics)
+
+## GPU Instance Type Availability
+
+HealthOmics supports the following GPU instance families: g4dn, g5, and g6. Not all GPU families are available in every region.
+
+Notable regional GPU limitations:
+- **il-central-1 (Tel Aviv)** — g5 only
+- **ap-southeast-1 (Singapore)** — g4dn only
+- **eu-west-1 (Ireland), eu-west-2 (London), and eu-central-1 (Frankfurt)** — g4dn and g5 only
+- **ap-northeast-2 (Seoul), ap-northeast-1 (Tokyo), and us-east-2 (Ohio)** — g4dn, g5, and g6 available. g5.12xlarge and larger sizes not currently available.
+
+## When to Use Each Region Type
+
+### Use Full-Feature Regions When:
+- You need to store genomic data in HealthOmics Storage (sequence stores, reference stores)
+- You need to query variant or annotation data using HealthOmics Analytics
+- You want to use Ready2Run sample workflows
+- You need all HealthOmics capabilities in one location
+
+### Use Workflows-Only Regions When:
+- You have custom workflows and manage data in S3
+- You want to keep compute close to other AWS resources in that region
+- You only need workflow execution without integrated storage or analytics
+
+## Related Documentation
+- For VPC subnet configuration and Availability Zone information, see [VPC Setup SOP](./vpc-setup.md)
+- For running workflows, see [Running a Workflow SOP](./running-a-workflow.md)
+- For workflow development, see [Workflow Development SOP](./workflow-development.md)
+- For ECR Pull-Through Cache setup, see [ECR Pull-Through Cache SOP](./ecr-pull-through-cache.md)
diff --git a/aws-healthomics/steering/vpc-setup.md b/aws-healthomics/steering/vpc-setup.md
index 8dc2fe0..1adfe9c 100644
--- a/aws-healthomics/steering/vpc-setup.md
+++ b/aws-healthomics/steering/vpc-setup.md
@@ -57,6 +57,12 @@ HealthOmics Workflows operates in the following Regions and Availability Zones.
 | ap-northeast-2 | ap-northeast-2a | apne2-az1 |
 | ap-northeast-2 | ap-northeast-2b | apne2-az2 |
 | ap-northeast-2 | ap-northeast-2c | apne2-az3 |
+| us-east-2 | us-east-2a | use2-az1 |
+| us-east-2 | us-east-2b | use2-az2 |
+| us-east-2 | us-east-2c | use2-az3 |
+| ap-northeast-1 | ap-northeast-1a | apne1-az4 |
+| ap-northeast-1 | ap-northeast-1c | apne1-az1 |
+| ap-northeast-1 | ap-northeast-1d | apne1-az2 |
 
 ## Procedure