diff --git a/src/coreclr/jit/valuenum.cpp b/src/coreclr/jit/valuenum.cpp
index e657b9dde198f5..55a450d2932478 100644
--- a/src/coreclr/jit/valuenum.cpp
+++ b/src/coreclr/jit/valuenum.cpp
@@ -2575,12 +2575,6 @@ ValueNum ValueNumStore::VNForFunc(var_types typ, VNFunc func, ValueNum arg0VN)
                 {
                     *resultVN = funcApp.GetArg(0);
                 }
-                // NOT(relop(x,y)) ==> Reverse(relop)(x,y)
-                //
-                else if (VNFuncIsComparison(funcApp.GetFunc()))
-                {
-                    *resultVN = GetRelatedRelop(arg0VN, VN_RELATION_KIND::VRK_Reverse);
-                }
             }
         }
 
diff --git a/src/tests/JIT/Regression/JitBlue/Runtime_129076/Runtime_129076.cs b/src/tests/JIT/Regression/JitBlue/Runtime_129076/Runtime_129076.cs
new file mode 100644
index 00000000000000..d593f7e4e35f5a
--- /dev/null
+++ b/src/tests/JIT/Regression/JitBlue/Runtime_129076/Runtime_129076.cs
@@ -0,0 +1,38 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+
+// VN was simplifying NOT(relop(x,y)) to Reverse(relop)(x,y). GT_NOT is
+// bitwise complement, not logical negation: ~(x relop y) produces -1 or
+// -2 for a 0/1 relop result, while Reverse(relop)(x,y) produces 0 or 1,
+// so downstream uses comparing the value arithmetically (here `~v3 >= -1`)
+// folded the wrong way.
+
+namespace Runtime_129076;
+
+using System.Runtime.CompilerServices;
+using Xunit;
+
+public static class Runtime_129076
+{
+    private static volatile int Input_p0 = unchecked((int)0x800335C5);
+    private static volatile int Input_p1 = 0;
+
+    [MethodImpl(MethodImplOptions.NoInlining)]
+    public static int Func(int p0, int p1)
+    {
+        unchecked
+        {
+            int v1 = unchecked((int)0x000335C5) & p1;
+            int v3 = (v1 != p0) ? 1 : 0;
+            if (v3 == 0) return 99;
+            int v4 = ~v3;
+            return (v4 >= -1) ? 1 : 0;
+        }
+    }
+
+    [Fact]
+    public static int TestEntryPoint()
+    {
+        return Func(Input_p0, Input_p1) == 0 ? 100 : 1;
+    }
+}
diff --git a/src/tests/JIT/Regression/Regression_ro_2.csproj b/src/tests/JIT/Regression/Regression_ro_2.csproj
index f8bedd81f9050b..82e13ec3df7e5c 100644
--- a/src/tests/JIT/Regression/Regression_ro_2.csproj
+++ b/src/tests/JIT/Regression/Regression_ro_2.csproj
@@ -100,6 +100,7 @@
     <Compile Include="JitBlue\Runtime_127446\Runtime_127446.cs" />
     <Compile Include="JitBlue\Runtime_128631\Runtime_128631.cs" />
     <Compile Include="JitBlue\Runtime_128801\Runtime_128801.cs" />
+    <Compile Include="JitBlue\Runtime_129076\Runtime_129076.cs" />
   </ItemGroup>
   <Import Project="$(TestSourceDir)MergedTestRunner.targets" />
 </Project>